Lattice Light-Sheet Microscopy as a Key Method for the Analysis of the Mislocalization of TDP-43 in Amyotrophic Lateral Sclerosis

Amyotrophic lateral sclerosis (ALS or Lou Gehrig’s disease) is a neurodegenerative disorder that characterized by the progressive loss of motor neurons. Consequently, patients afflicted with this disease eventually lose function associated moving, speaking, eating, and even breathing. One neuroscience team identified common mechanism in nearly all sporadic ALS which transactive response DNA binding protein 43 kDa (TDP-43) was mislocalized from nucleus to cytoplasm. This suggests mislocalization TDP-43 into cytoplasm central for onset patients, but underlying factors result process are not fully understood as there no current laboratory methods study progression mislocalization. However, development refinement lattice light-sheet microscopy (LLSM) technique has significant potential helping scientists elucidate unknown processes behind ALS. review discusses implications its optimization solution issue identifies LLSM key method could be field research.